Open Access Open Access  Restricted Access Subscription or Fee Access

Evaluation of Anticancer and Antibacterial Activity of Shpaeranthus Amaranthoides

L. Swarna Latha, P. Neelakanta Reddy

Abstract


Aim: the aim of the present study is to evaluate the antibacterial and anticancerous activity of Sphaeranthus amaranthoides. Methods: the elutes of the column chromatography by using ethylacetate and ethanol of Sphaeranthus amaranthoides were tested against multi drug resistant pathogenic bacteria they are E.coli ATCC 25922, Staphylococcus aureus ATCC 25923 and pseudomonal aeroginosa 27853. by using agar well diffusion method and MIC. The cytotoxic effect of the fraction on Hep2 cells were assayed using 3-(4,5-Dimethylthioazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT). Results: among the 3 organisms tested the extract showed a greater activity on pseudomonas then on staphylococcu aureus followed by E.coli. in both, agar well diffusion method and MIC. The 1:8 dilution of the ethylacetate fraction was non toxic to normal cells and also had both anticancerous and antiproliferative activities against cancerous cells. Conclusion: the study confirm the antibacterial and anticancerous activities of Sphaeranthus amaranthoides extract and is hence therefore a potential drug that requires further studies and development.

Keywords


Sphearanthus Amaranthoides, Antibacterial Activity, Anticancerous Activiy, HEp2 Cells, MTT Assay.

Full Text:

PDF

References


Nostro A, Germano MP, DÁngelo V, Cannatelli MA (2000). Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett. Appl. Microbiol. 30: 379-384.

Edeoga HO, Okwu DE, Mbaebie BO (2005). Phyto chemical constituents of some Nigerian medicinal plants. Af.r .J Biotechnol. 4: 685-688.

Haslam E. Plant Polyphenols. In: Haslam E (eds). Vegetable Tannins, Cambridge, England: Cambridge University Press, pp.15-89.

Scalbert A. Antimicrobial properties of tannin. Phyto chemistry, 1991; 30: 3875-3883.

Chung KT, Wong TY, Wei Y, Huang YW, Lin Y. Tannins and human health. A review. Crit Rev Food Sci Nutr, 1998; 8: 421-464.

Bisignano G, Sanogo R, Marino A, Aquino R, D'Angelo V, Germanò MP, De Pasquale R and Pizza C (2000) Antimicrobial activity of Mitracarpus scaber extract and isolated constituents. Lett. Appl. Microbiol. 30, 105-108.

Hammer KA, Carson CF and Riley TV (1999) Antimicrobial activity of essential oils and other plant extracts J. Appl. Microbiol. 86, 985-990.

Diallo D, Hveem B, Mahmoud MA, Betge G, Paulsen BS, Maiga A. An ethnobotanical survey of herbal drugs of Gourma district, Mali. Pharm Biol, 1999; 37: 80-91.

Rojas JJ, Ochoa VJ, Ocampo SA, Munoz JF. Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: A possible alternative in the treatment of non-nosocomial infections. BMC Complement Altern Med, 2006; 6: 2.

Erdogrul OT. Antibacterial activities of some plant extracts used in folk medicine. Pharm Biol, 2002; 40: 269-273.

Geissman TA. Flavonoid compounds, tannins, lignins and related compounds. In: Florkin M and Stotz EH (eds). Pyrrole Pigments, Isoprenoid Compounds and Phenolic Plant Constituents, New York, USA: Elsevier Press, 1963, pp 265.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev, 1999; 12: 564-582.

Michaud DS, Feskanich D, Rimm EB. Intake of specific carotenoids and risk of lung cancer in two prospective U.S. cohorts. Am J Clin Nutr, 2000; 72: 990-997.

Greenberg ER, Baron JA, Tosteson TD. A clinical trial of antioxidant vitamins to prevent colorectal cancer. N Engl J Med, 1994; 331: 141-147.

Birt DF, Hendrich S, Wang WQ. Dietary agents in cancer prevention: flavanoids and isoflavonoids. Pharmacol Ther, 2001; 90: 157– 177.

Morse MA, Stoner GD. Carcinogenesis,1993; 14: 1737.

Meyers KJ, Watkins CB, Pritts MP, Lin RH. Antioxi dant and antiproliferative ativities of strawberries. J.Agric.Food Chem, 2003; 51:6887-6892.

Wiseman, LR, Spencer CM (1998). Drugs aging 12: 305-334.

Hersey P Zhang XD. How melanoma cells evade trail-induced apoptasis. Nat.Rev.Cancer, 2001; 1: 142-150.

Taraphdar AK Roy R Bhattacharya RK. Natural products as inducers of apoptosis: Implication for cancer therapy and prevention. Curr.Sci, 2001; 80: 1387-1396.

Poncet KM Kroemer G. Chemotheraphy targeting the mitochondrial cell death pathway.2002.

Chellaram C and Edward JKP (2009) Anti-inflammatory potential of coral reef associated gastropod, Drupa margariticola. Indian J. Sci.Technol. 2 (2), 75-77. Domain site: http://www.indjst.org.

Rehan Ahmad, Swayam Prakash Srivastava, Rakesh Maurya, Rajendran SM, Arya KR and Arvind K. Srivastava (2008) Mild antihyperglycaemic activity in Eclipta alba, Berberis aristata, Betula utilis, Cedrus deodara, Myristica fragrans and Terminalia chebula. Indian J. Sci.Technol. 1 (5), 1-6.

NCCLS. Methods for Dilution Antimicrobial Suscepti bility Tests for Bacteria That Grow Aerobically; Approved Standard Fifth Edition. NCCLS document M7-A5. NCCLS: Wayne, PA, USA, 2000.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 1983; 65: 55-63.

Mishra B.B., Yadav S.B., Singh R.K., Tripathi V. A Novel Flavonoid C-glycoside from Sphaeranthus indicus L. (Family Compositae). Molecules. 2007; 12(10):2288-2291.

Heinrich M, Bremner P. Ethnobotany and ethnopharmacy – their role for anti-cancer drug development. Curr. Drug Targets, 2006; 7: 239- 245.

De Flora S, Ferguson L R. Overview of mechanisms of cancer chemopreventive agents. Mutat. Res, 2005; 591: 8-15.

Howells L M, Manson M M. Prospects for plant-derived chemopreventive agents exhibiting multiple mechanisms of action. Curr. Med. Chem. Anticancer Agents, 2005; 5: 201- 213.

Salah N, Miller NJ, Pagange G, Tijburg L, Bolwell GP, Rice E, Evans C. Polyphenolic flavonoids as scavenger of aqueous phase radicals as chain breaking antioxidant. 1995.

Del-Rio A, Obdululio BG, Castillo J, Martin FG, Orluno A. Uses and properties of Citrus flavonoids. J.Agric.Food.Chem, 1997; 45: 4505-4515.

Okwu DE. Phytochemicals and vitamin content of indigenous spices of south Eastern Nigeria. J. Susstain Agric.Environ, 2004; 6: 30- 34.

Bravo L. Polyphenols: Chemistry, dietary sources, metabolism and nutritional significance. Nutr.Rev., 1998; 56: 317-333.


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.