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The Bioactive Nature of the Marine Resources for Drug Discovery in Fish Networks

Thomas Stergiopoulos, Chenghao Bu

Abstract


This research due to the bioactive nature of the marine resources especially the echinoderms. Zebrafish from mixed sex populations were exposed continuously across three generations to two phytosterol preparations both containing-sitosterol. The phytosterols were isolated from wood and soy beans. Blood vitellogenin levels and sex ratio changes were used as intermediate indicators of the reproduction failures. Both sterol preparations caused vitellogenin induction in the exposed fish. The wood sterol changed the sex ratio of the exposed fish. In generation F1, the predominant sex was male, and in generation F2 it was female. The soy sterol in the used test concentration was lethal to the exposed fish in generation F1. This multigeneration test evidenced that phytosterols containing -sitosterol disrupt the reproduction system of zebrafish by changing the sex ratios and by inducing the vitellogenin production in the exposed fish. To assess human risk the initial assessment of these compounds is necessary. Zebra fishes are used in drug discovery development and it can be a constructive and cost-effective. This paper deals with the effect of sterol isolate from echinoderm on zebra fish (danio rerio). The selected active crude was characterized by spectroscopic analysis which is subjected to purification. The absorption frequency of FTIR and NMR is same as the absorption frequency of steroidal compound. The analyzed compound was tested for toxicity using the danio rerio. 50 mg/ml, 100 mg/ml, 200 mg/ml and 400 mg/ml are the four different concentrations where the fishes exposed. DNA fragmentation revealed that the DNA bands is not genotoxic to the DNA of fish.

Keywords


Secondary Metabolites, Stellaster Equestris, Zebra Fish, Sterol, NMR, FTIR, Toxicity Assessment, Compound A.

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