Open Access Open Access  Restricted Access Subscription or Fee Access

Effect of Certain Post Harvest Treatments on Elimination of Phytohaemagglutinins in Sword Bean (Canavalia Gladiata (Jacq DC.))

K.N. Rajnish, K. Janardhanan


Seed samples of Sword bean (Canavalia gladiata)
(Jacq ,DC) were analysed for phytohaemagglutinating (lectin)
activity. The lectins of raw seed samples exhibited lectin activity
without any specificity against human “A”, “B”, and “O”
erythrocytes. Among the different post harvest treatments almostcomplete reduction in phytohaemagglutinating activity was recordedwhen the seed samples were subject to autoclaving followed by dry heat treatment (roasting). Nonetheless, soaking in salt (NaCl) solution was found to be ineffective in reducing lectin activity.


Sword Bean, Phytohaemagglutinins, Soaking, Cooking, Autoclaving, Dry Heating (Roasting

Full Text:



Janardhanan, K, Vadivel, V and Pugalendhi, M 2003. Biodiversity in

Indian unexploited/tribal pulses. In: Biotechnology for the Improvement

of Legumes (Eds) Jawal P.K and Singh, R.P. Kluwer academic

publishers, Dordretch/ Boston/London.

Bressani, R and Elias, L.G, 1980. Nutritional value of legume crops for

humans and animals. In: Advances in legume Science. (Ed.)

Summerfield R.J and Bunting, A.H . pp. 135-155. Kew. Richmond,

Surrey, UK Royal Botanic Gardens.

Liener, I.E (1994). Implications of antinutritional components in

soybean foods Crit. Rev.Food. Sci. Nutr. 34: 31-67.

Liener , I.E., (1976). Phytohaemagglutinins (Phytolectins) Ann. Rev.

Plant. Physiol. 27: 291-319.

Pusztai, A., Ewen, S.W.B., Grant G., Peumans, W.J. van Damme E.J.M,

Rubio L. and Bardcoz, S. (1990). Relationship between survival and

binding of plant lectins during small intestinal passage amd their

effectiveness as growth factors. Digestion, 46: p 308.

Jindal, S., Soni, G.L., and Singh., R (1984) Biochemical and

histopathological studies in albino rats fed on soybean lectin. Nutr. Rep.

Int. 29: p95

Ishiguro. MN, Nakashima, Tanabe, S and Sakakaibara., (1992).

Biochemical studies on oral toxicity of ricin III. Intercation of toxic

lectin with epithelial cells of rat intestine, in vitro, Chem. Pharm, Bull,

Tokyo 40: p.441

Janardhanan, K and Gurumoorthy, P. Velvet bean- as a source of food,

feed and pharmaceuticals. Wisdom Publishers, Chennai, India (in press).

Duhan, A., Chauhan, B.M., Punia, D. and Kapoor, A.C. (1989). Phytic

acid content of chickpea (Cider arietinum) and black gram (Vigna

mungo): Varietal differences and effect of domestic processing and

cooking methods. J. Sci. Food Agri. 49: 449-455.

Almeida, N.G. Calderon de la Barca, A.M. and Valencia M.E. (1991).

Effect of different heat treatments on the anti-nutritional activity of

Phaseolus vulgaris (variety ojode cabra) lectin J. Agric Food Chem. 39:


Tan, N.H., Rahim, Z.H.A., Khor, H.T and Wong, K.C. (1983). Winged

bean (Psophocarpus tetragonolobus) tannin level, phytate content and

haemagglutinating activity. J.Agric. Food Chem 31: 916-917.

Siddhurju, P., Vijayakumari, K. and Janardhanan, K (1992). The

biochemical composition and nutritional potential of the tribal pulse,

Alysicarpus rugosus (Willd) DC. Food Chem 45: 251-255.

Vijayakumari, K., Siddhuraju., P and Janardhanan, K. (1994).

Nutritional assessment and chemical composition of the less known tree

legume, Acacia leucophloea (Roxb). Willd. Food Chem, 50: 285-288.

Vijayakumari, K., (1994). Studies on the antinutritional factors in tribal

pulses. Ph.D Thesis, Bharathiar University, Coimbatore, India.

Batra, V.I.P. (1981). Effect of cooking and germination on

haemagglutinating activity in lentil. Indian J.Nutr. Dietet, 24: 15-19.

Lowry, O.H, Rosebrough, N.J, Farr, A.L, and Randall R.J (1951) Protein

measurement with folin-phenol reagent. J. Biol. Chem. 193: 265-275.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.