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Artemia Toxicity of Marine Bacteria from Tuticorin Coastal Waters

Dr. T. Prem Anand, Dr. C. Chellaram


The discovery of novel chemical classes has been in decline for the past two decades, the need to exploit new resources in search for effective chemicals with novel mechanism of actions is imperative. Marine bacteria are such a resource yet to be tapped, and the potential it offers is vast. The antitumour assay (artemia toxicity bioassay) developed by McLaughlin et al. is being used as a "bench top bioassay" by many researchers for the preliminary screening of bioactive compounds. McLaughlin group has founded positive correlation between brine shrimp toxicity and cytotoxicity and has isolated a number of novel antitumor natural products using this bioassay. The lowest ED50 values were exhibited by 4 strains out of the 94 strains screened. Bacterial extracts exhibiting artemia toxicity test.


Antitumor Activity, Marine Bacteria, Gulf of Mannar.

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Ando, K., Oishi, H., Hirano, S., Okutomi, T., Suzuki, K., Okazaki, H., Sawada, M., and Sagawa, T., 1971. Tetranactin, a new miticidal antibiotic I, Isolation, characterization and properties of tetranactin. J. Antibiotics, 24 : 347-352.

Chimeno, R.I.F., Canedo, L, Espliego, F., Gravalos, D., Calle, F.D.L., and Puentes, J.L.F., 2000. 1B-96212 a novel cytotoxic macrolide produced by a marine Micromonospora. I. Taxonomy, Fermentation, Isolation, and Biological activities. J.Antibiotics, 53 (5): 474-478.

Dobler, I.W., Beil, W., Long, S., Meiners, M., and Laatsch, H., 2002. Integrated approach to explore the potential of marine microorganisms for the production of bioactive metabolites, Ad. Bio. Eng., Biotech special edition "Tools and applications of biochemical engineering". 74:207-238.

Fenical, W., and Jensen, P.R., 1993. Marine microorganisms: a new biomedical resource, Marine Biotechnology, Vol I, Pharmaceutical and Bioactive natural products Ed. Attaway, D .H., Zaborsky, O. R., New York, Plenum Press. 500.

Gailliot, F.P., 1998. Initial extraction and product capture, In : Methods in Biotechnology Vol.4 Natural Products Isolation, (ed) R.J.P., Cannell, Humana Press, NJ, USA, 53-89.

Gaustafson, K., Roman, M., and Fenical, W., 1989. The macrolactins a novel class of antiviral and cytotoxic macrolides from a deep-sea marine bacterium. J. Am. Chem. Soc, 111:7519-7524.

Imamura, N., Nishijima, M., Takadera, T., Adachi, K., Sakai, M., and Sano, H., 1997. New anticancer antibiotics pelagiomicins produced by a new marine bacterium Pelagiobacter variables. J.Antibiotics, 50 : 8-12.

Kennett,J.P.,1990.Introduction,In: Marine pharmacology, Prospects for the 1990, California Sea Grant workshop report No.T-CSGCP-022, university of California. La Jolla, California, 9-10.

Mc Laughlin, J.L., Chang, C.J., and Smith, D.L., 1988. Simple bioassays for the detection and isolation of bioactive natural products: In Plantes medicinals et Substances D'Origine Naturalle (ed) Delaveau, Universite D Angers, Angers 225-252.

Mc Laughlin, J.L., Chang, C.J., and Smith, D.L., 1991. "Bench-top" bioassays for the discovery of bioactive natural products; an update, In : Studies in natural products chemistry vol.9, (ed) Atta-ur-Rahman, Elsevier, Amsterdam, 383-407.

Munro, M.H.G., Blunt, J.W., Dumdei, E.J., Hickford, S.J.H., Lill, R.E., Li, S., Batters Hill, N., and Duckworth, A.R., 1999. The discovery and development of marine compounds with pharmaceutical potential. J. Biotechnol., 70 :15-25.

Okami, Y., Horta, K., Yoshida, M., Ikeda, D., Kondo, S., and Umezawa, H., 1979. New aminoglycoside antibiotics, istamycins A and B. J Antibiotics, 32 : 964-966.

Okami, Y., Okazaki, T., Kitahara, T., and Umezawa, H., 1976. Studies on marine microorganisms V. A new antibiotic, a plasomomycin, produced by streptomycete isolated from shallow sea mud. J.Antibitoics, 29:1019 - 1025.

Proksch, P., Edrada, R.A., Ebel, R., 2002. Drugs from the sea - current status and microbiological implications. Appl. Microbiol. Biotechnol., 59:125-134.

Sato, K., Okazaki, T., Maeda, K., and Okami, Y., 1978. New antibiotics aplasmomycins B and C. J.Antibiotics, 31 : 632.

Takahashi, A., Kurasawa, S., Ikeda, D., Okami, Y., and Takeuchi, T., 1989. Altemicidin, a new acarieidal and antitumor substance I, Taxonomy, fermentation, isolation and physico-chemical and biological properties. J.Antibiotics 42 :1556-1561.

Tan, L.T., Okino, T., and Gerwick, W.H., 2000. Hermitamides A and B, toxic malyngamide type natural products from the marine cyanobacterium Lyngbya majuscula. J.Nat.Prod. 63, 952-955.

Umezawa, H., Okami, Y., Kurasawa, S., Ohnuki, T., and Ishizuka, M., 1983. Marinactan, antitumor polysaccharide produced by marine bacteria. J.Antibiotics 36: 471 – 477.


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